M. Movahedi; V. Ghasemiomran; S. Torabi
Abstract
The present study was carried out to investigate the possibility of micro-propagation and to determine the optimal medium composition and combination of Cannabis sativa L. growth regulators under in vitro conditions. Seeds were surface-sterilized and then cultured on MS basal medium. One month later, ...
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The present study was carried out to investigate the possibility of micro-propagation and to determine the optimal medium composition and combination of Cannabis sativa L. growth regulators under in vitro conditions. Seeds were surface-sterilized and then cultured on MS basal medium. One month later, leaf and hypocotyl explants, obtained from the seedlings grown at in vitro condition, were used in MS culture medium containing NAA hormone (0.5, 1, 2 and 3 mg/l) either alone or in combination with 0.5mg/l BA; and 2,4-D (0.1, 0.2, 0.5 and 1 mg/l) alone or in combination with 0.5mg/l BA. Callus formation was the response of explants in most media. Direct shoot regeneration from explants was not observed but shoot induction from callus was seen only in 0.1 mg/L 2,4-D+ 0.5 mg/L BA. The highest volume of induced callus was formed on MS medium 0.1 mg/L 2,4-D+ 0.5 mg/L BA using leaf as explant. Root induction from some explants was observed in different treatments. The highest fresh weight of calli belonged to the leaf explant cultured on MS medium containing 1 mg/L 2,4-D+ 0.5 mg/L BA. Callus induction and rooting occurred easily and the explants did not respond well to regeneration.
M. Raee; M. Omidi; S. Torabi; M. Khidayari
Abstract
Due to the exclusively biological and physicochemical characteristics of nanoparticles, they are widely used in biology and agriculture. Currently, silver nanoparticles (Nano-Ag) have wide application in nanotechnology. Nano-TiO2 as a semiconductor metal oxide nanocrystal has a special position in industrial ...
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Due to the exclusively biological and physicochemical characteristics of nanoparticles, they are widely used in biology and agriculture. Currently, silver nanoparticles (Nano-Ag) have wide application in nanotechnology. Nano-TiO2 as a semiconductor metal oxide nanocrystal has a special position in industrial world. The mentioned nanoparticles were used in cell culture of Aloe vera. In this study, the effects of nano-elicitors including Nano-Ag and Nano-TiO2 were investigated on cell suspension culture of Aloe vera. Manipulation of cell culture media by abiotic elicitors is an important way for inducing production of valuable metabolites. Aloin is the major anthraquinone compound found in Aloe vera. It has antimalarial, antifungal, antibacterial, and antiviral properties. The induced callus by elicitors was collected in five periods and analyzed by HPLC (High-performance liquid chromatograghy). Results showed that Nano-Ag caused to increased content of aloin to 43.7% within 48 hours and then this amount was reduced to the control level. Nano-Tio2 caused to increased content of aloin to 11.6% in 48 hours after elicitation; however, 168 h after treatment, it was reduced to 8.8% as compared to the control. Results suggest that nanoelicitors can regulate the production of Aloin in Aloe vera.
S. Shamsian; M. Omidi; S. Torabi
Abstract
Aloe (Aloe vera L.) is one of the most valuable plants in the pharmaceutical, cosmetic, food and health industries. Due to the growing need for this plant, extensive research has been done on in vitro culture. In vitro propagation of this plant is a convenient and efficient way for the massive proliferation ...
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Aloe (Aloe vera L.) is one of the most valuable plants in the pharmaceutical, cosmetic, food and health industries. Due to the growing need for this plant, extensive research has been done on in vitro culture. In vitro propagation of this plant is a convenient and efficient way for the massive proliferation and the use of secondary metabolites. In this study, the best method of sterilization for the explants derived from Aloe vera seedlings and the effects of growth regulators BAP, NAA, and activated charcoal were investigated. The experiment was based on a completely randomized (CRD) design with 3 replications and 5 treatments. BAP at concentrations of 5 and 4 mg per liter, NAA at 0.2 mg per liter, and activated charcoal at 2 grams per liter were used in the MS-medium to induce the lateral buds of seedlings. Fungicide carbendazim 1% (20 min.), alcohol 70% (30 seconds), sodium hypochlorite 2.5% (20 min.), mercuric chloride 0.1% (10 minutes) was identified as the best protocol. In addition, MS-medium with 5 mg/L BAP and 2 grams per liter of activated charcoal was known to be the best environment to induce the lateral buds. The buds obtained in the MS-medium containing 2.0 ppm NAA were rooted and 85% of cuttings survived in the greenhouse.
M. Movahedi; V. Ghasemiomran; S. Torabi
Abstract
Cannabis sativa L., belonging to cannabinaceae family, is a medicinal plant that produces a diverse array of secondary metabolites. The present study aimed to optimize in vitro direct and indirect regeneration in this valuable plant. For this reason, leaf and hypocotyl explants from in vitro grown plantlets ...
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Cannabis sativa L., belonging to cannabinaceae family, is a medicinal plant that produces a diverse array of secondary metabolites. The present study aimed to optimize in vitro direct and indirect regeneration in this valuable plant. For this reason, leaf and hypocotyl explants from in vitro grown plantlets were excised and cultured on MS media containing different concentrations of BA (0.1, 0.2, 0.5, 1, 2 and 3mg/l) and TDZ (0.1, 0.2, 0.5, 1, 2 and 3mg/l) alone or in combination with 0.5 mg/l IBA. The explants reaction in all media led to formation of calli. Direct shoot regeneration from explants was not observed but shoot induction from callus was seen on MS media containing different concentrations of BA in hypocotyl explants. The highest volume of callus was formed on MS medium containing 2 mg/l TDZ+ 0.5mg/l IBA using leaf as explant. The highest wet and dry weight of callus belonged to leaf explant cultured on MS medium containing 1 mg/l TDZ+ 0.5 mg/l IBA. MS medium containing 0.5 mg/l TDZ+ 0.5 mg/l IBA produced no callus in both explants. The addition of IBA in different concentration of BA had significant effects on volume, wet and dry weight of calli in both explants whereas it had no significant effects in different concentrations of TDZ.
